The Cohen-Karni Lab has been exploring numerous biosensing research thrusts such as dopamine sensing using our three-dimensional fuzzy graphene (3DFG) electrodes. The use of 3DFG electrodes has allowed multi-site detection of dopamine with high spatial and temporal resolution.
3DFG Dopamine Sensing
3DFG electrodes have been used to demonstrate dopamine sensing with a sensitivity of 2.12 ± 0.05 nA/nM and 0.036 ± 0.001 nA/nM for 50 x 50 μm and 2 x 2 μm microelectrodes, respectively. Use of 3DFG electrodes demonstrated sensitivity 162-432 fold greater than CFEs, PEDOT/Nafion CFEs, and PEDOT/GO CFEs with 2200 fold increase in geometric area due to the high surface area of 3DFG.
3DFG electrodes have been used to simultaneously detect DA and 5-HT (serotonin) using FSCV. 3DFG electrodes were able to differentiate the oxidation and reduction peaks of DA and 5-HT unlike CFEs, this difference being attributed to the higher sensitivity and extremely high surface area of 3DFG electrodes.
